rabbit anti rbfox1  (Novus Biologicals)

Journal: bioRxiv

Article Title: Correction of RBFOX1 deficit rescues Huntington’s disease mis-splicing and pathology

doi: 10.1101/2024.11.06.622223

Figure Lengend Snippet: ( A ) Normalized counts of the three RBFOX genes in striatal RNA from 3.5 month-old WT (n=3) and R6/1 mice (n=3) according to RNA-seq datasets in Elorza et al . 15 ( B ) Quantification of Rbfox1 transcript levels by RT-qPCR in striatal RNA from 3.5 month-old WT (n=7) and R6/1 mice (n=7) (Student’s t-test; * P < 0.05). ( C ) Rbfox1 immunohistochemistry in striatum of 3.5 month-old WT and R6/1 mice. ( E ) Representative RBFOX1 immunohistochemistry staining in striatum of control and HD subjects.

Article Snippet: Antibodies: rabbit anti-RBFOX1 (1:5000, Novus, NBP1-90304), rabbit anti-β-Gal (1:2000, Invitrogen, A-11132), rabbit DARPP32 (1:3000, BD, 611520) and rabbit Cleaved caspase-3 (1:100, Cell Signaling, 9661) for mouse samples.

Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Immunohistochemistry, Staining, Control

Journal: bioRxiv

Article Title: Correction of RBFOX1 deficit rescues Huntington’s disease mis-splicing and pathology

doi: 10.1101/2024.11.06.622223

Figure Lengend Snippet: ( A ) Mice expressing tTA under control of the CamKII promoter (CamKII-tTA mice) were bred with mice carrying the β-Gal-BiTetO-RBFOX1 or β-Gal-BiTetO-U2AF2 construct to yield TgRBFOX1 (CamKII-tTA:β-Gal-BiTetO-RBFOX1) mice or TgU2AF2 (CamKII-tTA:β-Gal-BiTetO-U2AF2) . ( B ) Immunohistochemistry with anti-RBFOX1 or anti-U2AF2 antibody in sagittal sections from 1.5 month-old WT, TgRBFOX1 or TgU2AF2 mice.

Article Snippet: Antibodies: rabbit anti-RBFOX1 (1:5000, Novus, NBP1-90304), rabbit anti-β-Gal (1:2000, Invitrogen, A-11132), rabbit DARPP32 (1:3000, BD, 611520) and rabbit Cleaved caspase-3 (1:100, Cell Signaling, 9661) for mouse samples.

Techniques: Expressing, Control, Construct, Immunohistochemistry

Journal: bioRxiv

Article Title: Correction of RBFOX1 deficit rescues Huntington’s disease mis-splicing and pathology

doi: 10.1101/2024.11.06.622223

Figure Lengend Snippet: (A) Gel shows RT-PCR amplification of Tg-RBFOX1 or TgU2AF2 mRNA in wild-type, MildTgRBFOX1 or MildTgU2AF2 and StrongTgRBFOX1 or StrongTgU2AF2 mice. (B) Histogram shows brain weight of WT (n=5/n=8), MildTgRBFOX1 (n=7) or MildTgU2AF2 (n=5) and Strong TgRBFOX1 (n=7) or Strong TgU2AF2 (n=6) mice (ANOVA, followed by Tukey’s post hoc test; *P < 0.05;**P < 0.01; ***P < 0.001).

Article Snippet: Antibodies: rabbit anti-RBFOX1 (1:5000, Novus, NBP1-90304), rabbit anti-β-Gal (1:2000, Invitrogen, A-11132), rabbit DARPP32 (1:3000, BD, 611520) and rabbit Cleaved caspase-3 (1:100, Cell Signaling, 9661) for mouse samples.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: bioRxiv

Article Title: Correction of RBFOX1 deficit rescues Huntington’s disease mis-splicing and pathology

doi: 10.1101/2024.11.06.622223

Figure Lengend Snippet: ( A ) Venn diagram showing the 83 genes in the intersection between the 245 genes with exons mis-spliced in Huntington’s disease 15 and the 543 genes with RBFOX-direct target exons 25 ( B ) Venn diagram showing the 76 genes in the intersection between the 245 genes with exons mis-spliced in Huntington’s disease 15 and the 966 genes that are functional targets of Rbfox1 according to Supplementary Table 1. Representation factor (RF) was determined with Two-sided Fisher’s Exact test, using as background genes the human-mouse orthologous genes coincidentally detected in the human and mouse RNA-seq datasets used to define the Huntington’s disease mis-splicing signature 15 ( n = 12,882).

Article Snippet: Antibodies: rabbit anti-RBFOX1 (1:5000, Novus, NBP1-90304), rabbit anti-β-Gal (1:2000, Invitrogen, A-11132), rabbit DARPP32 (1:3000, BD, 611520) and rabbit Cleaved caspase-3 (1:100, Cell Signaling, 9661) for mouse samples.

Techniques: Functional Assay, RNA Sequencing Assay

Journal: Bioscience Reports

Article Title: Rbfox1 expression in amacrine cells is restricted to GABAergic and VGlut3 glycinergic cells

doi: 10.1042/BSR20220497

Figure Lengend Snippet: Primary and secondary antibodies used for immunohistochemistry

Article Snippet: Rbfox1 , Novus Biologicals , NBP2-13169 , Monoclonal , Mouse , 1:200.

Techniques:

Journal: Bioscience Reports

Article Title: Rbfox1 expression in amacrine cells is restricted to GABAergic and VGlut3 glycinergic cells

doi: 10.1042/BSR20220497

Figure Lengend Snippet: ( A ) In the GCL, all Rbpms-positive cells, RGCs, were also stained for Rbfox1 (yellow arrows point at some Rbpms/Rbfox1-positive cells). Red arrows point at some Rbfox1-positive/Rbpms-negative cells that are most likely displaced ACs. ( B ) In the INL, virtually all clabindin-positive cells were also Rbfox1-positive cells (yellow arrows). Very few Rbfox1-immunostained cells appeared to be calbindin-negative (red arrows). In the GCL, Rbfox1 is expressed in calbindin-positive displaced ACs (yellow arrows), as well as in calbindin-negative cells, many of which are RGCs (red arrows). ( C ) Colocalization of Rbfox1 expression with RGCs in whole mount retinas. All Rbpms-positive cells were immunostained with Rbfof1. Rbfox1-positive/Rbpms-negative cells (presumably displaced ACs) are indicated by white arrows. ( D ) A significant overlap in Rbfox1 and calbindin expression within GCL was observed in whole mount retinas (yellow arrows). Rbfox1-positive/calbindin-negative and calbindin-positive/Rbfox1-negative cells are pointed with red and green arrows, respectively. DAPI; 4',6-diamidino-2-phenylindole; GCL; ganglion cell layer, INL; inner nuclear layer, IPL; inner plexiform layer, ONL; outer nuclear layer, OPL; outer plexiform layer.

Article Snippet: Rbfox1 , Novus Biologicals , NBP2-13169 , Monoclonal , Mouse , 1:200.

Techniques: Staining, Expressing

Journal: Bioscience Reports

Article Title: Rbfox1 expression in amacrine cells is restricted to GABAergic and VGlut3 glycinergic cells

doi: 10.1042/BSR20220497

Figure Lengend Snippet: ( A ) Rbfox1 is expressed in very few GlyT1-positve ACs (yellow arrows). Rbfox1-positive/GlyT1-negative and GlyT1-positive/Rbfox1-negative cells are pointed with red and green arrows, respectively. ( B ) Rbfox1 is expressed in sparse population of VGlut3-positve glycinergic ACs (yellow arrows). Red arrows point at Rbfox1-positive cells that not immunostained for GlyT1 (A) or for VGlut3 (B). The boxed areas of the vertical retinal sections are shown at higher magnification.

Article Snippet: Rbfox1 , Novus Biologicals , NBP2-13169 , Monoclonal , Mouse , 1:200.

Techniques:

Journal: Bioscience Reports

Article Title: Rbfox1 expression in amacrine cells is restricted to GABAergic and VGlut3 glycinergic cells

doi: 10.1042/BSR20220497

Figure Lengend Snippet: ( A ) The vast majority GABAergic ACs in the INL were Rbfox1-positive. Some GABA/Rbfox2-positive ACs are pointed with yellow arrows. Rbfox1-negative GABAergic ACs and Rbfox1-positve/GABA-negative cells are pointed with green and red arrows, respectively. ( B ) Rbfox1 is expressed in all cholinergic (ChAT-positive) starburst ACs both in the INL and GCL. ( C ) Extensive overlap of Rbfox1 with NPY expression was also observed (yellow arrows). Very few NPY-positive cells had very faint staining for Rbfox1 (pointed by green arrows).

Article Snippet: Rbfox1 , Novus Biologicals , NBP2-13169 , Monoclonal , Mouse , 1:200.

Techniques: Expressing, Staining